Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59) (CAT#: PABC-523)
This product is a Mouse antibody that recognizes EBV gH/gL. CL59 can block membrane fusion with both B cells and epithelial cells. CL59 binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites, indicating the functional importance of this domain in fusion activation.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ELISA
Figure 3 gHgL mutant surface expression and conformation was monitored using anti-gHgL E1D1, CL40 and CL59 monoclonal antibodies and HL800 polyclonal serum using a cell ELISA assay.
The results are expressed as % change in antibody binding relative to wt, with most antibodies showing no changes in binding to the mutants relative to the wt gHgL (values near zero represent wt binding). E1D1 binding is selectively lost for mutations in gl residues gl:L74, glL:176 and glL:Y131. n.d., not detected. A western blot of the gi N69 glycosylation mutants detected using a polyclonal anti-gHgL antibody (rabbit) is shown adjacent to the antibody binding data, with monoclonal anti-GAPDH blots shown as a loading control. The gel shows the expected shift in molecular weight of the gl mutants due to loss of glycan at the mutated NXS motif.
Sathiyamoorthy, K., Hu, Y. X., Möhl, B. S., Chen, J., Longnecker, R., & Jardetzky, T. S. (2016). Structural basis for Epstein–Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. Nature communications, 7, 13557.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EBV gH/gL
- Species Reactivity
- EBV
- Clone
- CL59
- Applications
- ELISA, FC, Neut, Inhib
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Neutralization, Inhibition.
Target
- Alternative Names
- EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone CL59"
See other products for "EBV gH/gL"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-311 | Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) | ELISA, BLI, Neut | Human IgG |
| PABC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) | ELISA, Inhib, FC, Neut | Mouse IgG1 |
| PABC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1) | ELISA, FC, Neut, Inhib | Mouse IgG2a, κ |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABC-523. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-523, RRID: AB_3111597)
Copy citation
Popular products with customers
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application:
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, FC, Neut, Inhib
Application: ELISA, Neut
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
