Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59)
CAT#: PABC-523
This product is a Mouse antibody that recognizes EBV gH/gL. CL59 can block membrane fusion with both B cells and epithelial cells. CL59 binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites, indicating the functional importance of this domain in fusion activation.
Published Data
Tested Data
ELISA
Figure 3 gHgL mutant surface expression and conformation was monitored using anti-gHgL E1D1, CL40 and CL59 monoclonal antibodies and HL800 polyclonal serum using a cell ELISA assay.
The results are expressed as % change in antibody binding relative to wt, with most antibodies showing no changes in binding to the mutants relative to the wt gHgL (values near zero represent wt binding). E1D1 binding is selectively lost for mutations in gl residues gl:L74, glL:176 and glL:Y131. n.d., not detected. A western blot of the gi N69 glycosylation mutants detected using a polyclonal anti-gHgL antibody (rabbit) is shown adjacent to the antibody binding data, with monoclonal anti-GAPDH blots shown as a loading control. The gel shows the expected shift in molecular weight of the gl mutants due to loss of glycan at the mutated NXS motif.
Sathiyamoorthy, K., Hu, Y. X., Möhl, B. S., Chen, J., Longnecker, R., & Jardetzky, T. S. (2016). Structural basis for Epstein–Barr virus host cell tropism mediated by gp42 and gHgL entry glycoproteins. Nature communications, 7, 13557.
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SDS-PAGE
Figure 1 Recombinant Mouse Anti-EBV gH/gL Antibody (PABC-523) in SDS-PAGE
Lane M: Marker
Lane 1: PABC-523 in non-reduced condition.
Lane 2: PABC-523 in reduced condition.
SDS-PAGE
Figure 2 Recombinant Mouse Anti-EBV gH/gL Antibody (CL59) (PABC-523) in SDS-PAGE
SDS-PAGE analysis of PABC-523 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 3 Recombinant Mouse Anti-EBV gH/gL Antibody (CL59) (PABC-523) in SEC-HPLC
SEC-HPLC analysis of PABC-523 shows that the purity is more than 95%
WB
Figure 4 Recombinant Mouse Anti-EBV gH/gL Antibody (CL59) (PABC-523) in WB
Western blot analysis of PABC-523 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
ELISA
Figure 5 Recombinant Mouse Anti-EBV gH/gL Antibody (CL59) (PABC-523) in ELISA
ELISA analysis of PABC-523 was performed by coating with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
DB
Figure 6 Recombinant Mouse Anti-EBV gH/gL Antibody (CL59) (PABC-523) in Dot Blot
Dot Blot analysis of PABC-523 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag.
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EBV gH/gL
- Species Reactivity
- EBV
- Clone
- CL59
- Applications
- ELISA, FC, Neut, Inhib
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Neutralization, Inhibition.
Target
- Alternative Names
- EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-523, RRID: AB_3111597)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone CL59"
- CAT
- Product Name
See other products for "EBV gH/gL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-311 | Recombinant Human Anti-EBV gH/gL Antibody (AMMO1) | ELISA, BLI, Neut | Human IgG |
| PABC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody (CL40) | ELISA, Inhib, FC, Neut | Mouse IgG1 |
| PABC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody (E1D1) | ELISA, FC, Neut, Inhib | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-311 | Recombinant Human Anti-EBV gH/gL Antibody Fab Fragment (AMMO1) | ELISA, Neut | Human Fab |
| PFBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL40) | ELISA, FC, Neut | Mouse Fab |
| PFBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (CL59) | ELISA, FC, Neut | Mouse Fab |
| PFBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody Fab Fragment (E1D1) | ELISA, FC, Neut | Mouse Fab |
| PABC-523-F(E) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); Fab Fragment | ELISA, FC, Neut, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-311 | Recombinant Human Anti-EBV gH/gL Antibody scFv Fragment (AMMO1) | ELISA, Neut | Human scFv |
| PSBC-419 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL40) | ELISA, FC, Neut | Mouse scFv |
| PSBC-523 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (CL59) | ELISA, FC, Neut | Mouse scFv |
| PSBC-524 | Recombinant Mouse Anti-EBV gH/gL Antibody scFv Fragment (E1D1) | ELISA, FC, Neut | Mouse scFv |
| PABC-523-S(P) | Mouse Anti-EBV gH/gL Recombinant Antibody (clone CL59); scFv Fragment | ELISA, FC, Neut, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-XY12 | Human Anti-EBV gH/gL Vaccinal Antibody (VS-0625-XY12) | ELISA, Neut | Human IgG |
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